VariFind™ PULM Assay
NGS-based assay for for detection of clinically significant genetic variants in 83 genes associated with pulmonary diseases and related conditions

Intended use: molecular-genetic investigation of pulmonary diseases and related conditions
Disordes included
Acute respiratory distress
Spontaneous family pneumothorax
Pulmonary hypertension
Lymphangioleiomyomatosis
BPCO
Primary ciliary dyskinesia
Pulmonary fibrosis
Emphysema
Hereditary pulmonary alveolar proteinosis
Acute nocturnal respiratory distress
Pulmonary cysts
Cystic fibrosis
Bronchiectasis
Genes included
Assay information
Analyzed region
CDSs + 20 bp padding (except CFTR)
For CFTR: CDSs + UTRs
Target region size
469 kb
Platform
Illumina MiSeq Series
Assay workflow
NGS Libraries Preparation
Sequencing
Automated data analyis & annotation
Report generation
Sequencing conditions for Illumina
The recommended length cycling format for sequencing libraries prepared using VariFind™ Pulmonary Panel Solution v2 are 500 and 600-cycle run in paired-end mode.
Assay is compatible with Illumina™ MiSeq® Series Systems (MiSeq®, MiSeq® Dx, MiSeq® FGx in research mode).

MiSeq Reagent Kit v2
Cycles
Output
Output in reads
Reads per sample
Max. sample/run
500
400 + 100 bp
13 500 000
≈ 860 000
15

MiSeq Reagent Nano Kit v2
Cycles
Output
Output in reads
Reads per sample
Max. sample/run
500
400 + 100 bp
1 000 000
≈ 860 000
1

MiSeq Reagent Kit v3
Cycles
Output
Output in reads
Reads per sample
Max. sample/run
600
400 + 200 bp
23 500 000
≈ 860 000
27
Made on
Tilda