VariFind™ HID project news
On April 19, during the "Molecular Diagnostics 2017
" conference (Moscow) Vasiliy Pekhov (the employee of the department of medical and biological research of the organization of the forensic-criminal activity of the Main Criminalistics Department of the Investigative Committee of the Russian Federation) presented the results of molecular genetic expertise conducted by means of the VariFind™
HID solution developed by Parseq Lab.
HID is a complex solution for human identification based on high-performance sequencing technology designed to resolve cases where currently used capillary electrophoresis (CE) methods are not sufficient to obtain reliable results. This solution fits for analysis of low quality DNA samples, DNA mixtures from two or more contributors, stain samples in cases without any known suspects.
Vasily Pekhov reported about several examinations conducted by the Investigative Committee, where the VariFind™
HID solution was used to obtain additional information about samples.
In two examinations MPS technology was applied to deal with two-contributors DNA mixtures where minor contributor's genotype was especially interesting for investigation, but the minor component ratio was below the level acceptable for CE analysis.
Another example given in the report was the use of the VariFind™
HID solution to identify bone remains. In this case strongly degraded DNA was isolated from bone samples and then sequenced. Genetic profile obtained was sufficient for comparison with known relatives.
In addition, the solution was used in molecular-genetic expertise for phenotype prediction. Most probable suspect's eye and hair colors were estimated based on crime scene stain DNA samples.
Based on the completed work, it was concluded that the high-throughput sequencing method allows obtaining additional information in cases that cannot be resolved by traditional approaches and extends the capabilities of forensic DNA expertise.
HID solution includes reagent kits to examine a large number of specially selected loci, an infrastructure for data storage and management — Genome Server™
— and software for analysis of sequencing results, forensic hypothesis testing, and subsequent report generation. The solution consists of three modules that are designed for human identification, human ancestry estimation, and phenotype prediction.
Read more on the VariFind™ HID solution page