VariFind™ HLA Assay
NGS Assay for high-througoutput high-resolution human HLA typing

Intended use: Intermediate high (4 digit) resolution HLA typing of donors for Bone Marrow Donors Registries
Assay information
Gene coverage

CLASS I
A
B
C

CLASS II
DRB1
DQB1




CDSs with 5`-UTR and 3`-UTR
CDSs with 5`-UTR and 3`-UTR
CDSs with 5`-UTR


Exons 1-4 with 5`-UTR
Exons 2-4
Platform
Illumina MiSeq/MiniSeq Series
100 %
HLA-A
HLA-B
HLA-DQB1
HLA-C
HLA-DRB1
Matching genotypes
98,9 %
93,5 %
95,2 %
100 %
100 %
95,5 %
98,2 %
97,6 %
0,00 %
0,00 %
0,01 %
0,07 %
0,01 %
100 %
Matching allels
Ambiguity
Assay Performance
Clinical utility
Analytical validation
93 samples from registry
Typed 908 alleles
Clinical Validation
192 samples from registry
Typed 1880 alleles
Product composition
Reagents kit
Targeted enrichment , library preparation, molecular barcoding
VariFind™ Software for
MPS data analysis & automated allele assignment
Assay workflow
NGS Libraries Preparation
Sequencing
Automated data analyis
Report generation
Made on
Tilda